英语翻译As shown in Fig.1,a protein peak containing chitosanase

英语翻译
As shown in Fig.1,a protein peak containing chitosanase and
CMCaseactivities and two other protein peaks just withCMCase
activity were eluted from the ion-exchange chromatograph of
Sepharose CL-6B.Fraction with bifunction was collected,and
others were removed using this procedure.Hydrophilic chromatography
of the ion exchange purified bifunctional fraction
on Phenyl Sepharose CL-4B resulted in one protein peak (peak
2) with bifunction and two protein peaks just with chitosanase
(peak 1) or cellulase activity (peak 3) as shown in Fig.2.
Enzymes obtained from Phenyl Sepharose CL-4B were further
purified on a Sephadex-G-75 column and a major protein peak
was obtained as shown in Fig.3.The purification results are summarized
inTable 1.Through these steps,the enzymewas purified
3.16-fold with a recovery of 10.47%.The recovery of the enzyme
activity was rather low due to the removal of some minor activ-
ity peaks during chromatography or some of the activity losses
occurred during dialysis and lyophilization steps.Even though
the recovery fold is the lowest when using gel filtration,it is a
necessary method to change the enzyme buffer for further determination
enzyme activity.The purification fold of CMCase is
not in agreement with that of chitosanase,maybe owing to three
factors:the degrading CMC is a synergistic action of coenzyme
but most of the cellulase active portion was removed during
the purification procedure; the CMCase activity was determined
by 3,5-dinitrosalicylic acid method which only measuring the
reducing sugar; the bifunctional enzyme may has two different
active centers,although one enzyme possesses both activities.

如图所示.1,含有壳聚糖酶和蛋白峰
CMCaseactivities和另外两个蛋白质峰刚刚withCMCase
从活性洗脱离子交换色谱的
琼脂糖凝胶CL - 6B型.与双功能分数为收集和
其他被拆除使用此过程.亲水色谱法
离子交换纯化的双功能的一小部分
对苯基琼脂糖凝胶CL - 4B型导致1蛋白峰（峰
2）双功能蛋白峰和两个刚刚与壳聚糖
（峰1）或纤维素酶（峰3）,如图所示.2.
酶获得苯基琼脂糖凝胶CL - 4B的进一步
纯化上一经Sephadex - G - 75柱和主要的蛋白峰
得到如图所示.3.纯化结果摘要
inTable 1.通过这些步骤,enzymewas纯化
3.16倍与10.47％的回收率.这种酶的复苏
活动是相当低由于取消了一些轻微的活动唤醒
在色谱或活动的一些损失,两者均峰
发生在透析,冷冻干燥的步骤.即使
恢复倍是最低的使用凝胶过滤,它是一个
必要的方法来改变酶作进一步确定缓冲区
酶的活性.纤维素酶的纯化倍,是
不符合的壳聚糖酶,或许由于三协议
因素：有辱人格的CMC是一种辅酶协同行动
但是,纤维素酶的活动部分大部分被拆除时
净化程序;的CMC酶的活性测定
由3,5 -二硝基水杨酸法只测量
还原糖;的双功能酶可能有两种不同的
活跃的中心,尽管人们酶具有两种活动.