英语翻译在下感激不尽!一天内生效.IntroductionChloride channels may contribut

英语翻译
在下感激不尽!一天内生效.
Introduction
Chloride channels may contribute to the cardiac action potential,causing a depolarization of the resting membrane potential and a shortening of action potential duration.1 In this way,these channels are targets for antiarrhythmic drug therapy,for which an appreciation of such currents may allow for deeper insights into the physiological control of cardiac rhythm in humans.
Several types of cardiac chloride currents have been re¬ported,including a cAMP-regulated current,a Ca2+ -activated current,a swelling-activated current,and/or a current result¬ing from phosphorylation by protein kinase C (PKC) (for review,see reference 1).In human atrial cells,several stud¬ies have identified the presence of a swelling-activated chlo¬ride current,but failed to detect other chloride ion-mediated components.2-4 The anionic current displays strong outward rectification and blockade by disulfonic stilbenes.Neverthe¬less,the literature contains limited reports of Cl- currents in human atrial cells at the single-channel level.To our knowl¬edge,only one study reported the characteristics of a chlo¬ride channel in isolated human atrial cardiomyocytes.5 In that study,the channel was stretch-activated and presented a linear I/V relationship with a conductance of 9 pS.The bio¬physical properties of this anionic conductance diverge from the known properties of the swelling activated Cl- current,at least regarding its well-established outward rectification.The described channel would more likely correspond to the PKC-activated Cl- channel.6
The purpose of the present experiments was to determine whether other single Cl- channel currents can be identified in inside-out membrane patches from human atrial myocytes and,if so,to establish the biophysical properties of these channels and their responses to disulfonic stilbene Cl- trans¬port blockers.
We describe here the properties of an outwardly rectify¬ing Cl- channel in freshly isolated human atrial cardiomy- ocytes and its blockade by disulfonic stilbenes.In addition,we showed that the activity of this channel is upregulated under PKC activation and cell swelling.

介绍
氯离子通道可能有助于心脏的动作电位,引起去极化静息膜电位和行动,这种方式的潜在duration.1缩短,这些渠道是抗心律失常药物治疗的目标,而这种电流的升值可能允许在人类心脏节律的生理控制更深的了解.
几种心脏氯电流已重新¬移植,包括营,调节电流,1 +激活钙电流肿胀激活的电流,和/或1当前结果¬从磷酸ING蛋白激酶Ç（PKC）（适用于审查,见参考文献1）.在人类心房细胞,几个螺柱¬IES已确定的膨胀激活chlo¬乘坐电流的存在,但未能发现其他氯离子介导的components.2-4阴离子当前显示强大的外向整流和苯乙烯二磺酸封锁.尽管如此¬少,文献包含CL-电流有限的报道,在人类的心房细胞,在单声道级别.就我们所知¬边缘,只有一个研究报告的chlo¬搭在这项研究中孤立的人类心房cardiomyocytes.5通道的特点,渠道牵张激活,并提出了I / V的线性关系,与9 PS导. ¬本阴离子电导的生物物理性质,化学性质,偏离肿胀激活CL-目前已知的属性,至少就其既定的外向整流.所描述的通道,将更有可能对应PKC激活CL-channel.6
本实验的目的是确定是否可以从人类心房肌细胞内而外膜补丁确定其他单一的氯离子通道电流,如果是这样,建立这些渠道,他们的反应的生物物理特性二磺酸苯乙烯CL-反¬端口阻滞剂.
在这里,我们描述一个外表纠正的属性¬ING CL-通道在新鲜分离的心房人类cardiomy ocytes和二磺酸苯乙烯的封锁.此外,我们发现,这个通道的活性上调PKC的激活和细胞肿胀下.